Surgical Cannulation Technique
Surgical Cannulation Technique - Related Videos
05:18
Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct
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Cited by 17 •
2011
A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.
02:05
Intraduodenal Cannulation in a Rat Model for Immunopathology Studies
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2025
This video demonstrates intraduodenal cannulation in a rat model. After anesthetizing the rat, the duodenum is located and a small puncture is created. A J-shaped cannula with a beveled tip is carefully inserted and secured, and the incision is closed. The cannulated rat model enables intra-intestinal infusion for immunopathology studies.
05:05
Locating the Cisterna Magna in a Pig Model: A Surgical Method to Access the Cisterna Magna in a Pig Model for Direct Cannulation
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2025
In this video, we describe a method to access the cisterna magna in a pig model. The cisterna magna is a preferred site for studies concerning cerebrospinal fluid.
04:28
Cannulation of the Carotid Artery in a Rat Model
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2025
In this video, we demonstrate a surgical procedure to cannulate the left carotid artery in a rat model.
09:49
Isolation and Cannulation of Cerebral Parenchymal Arterioles
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Cited by 28 •
2016
This manuscript describes a simple and reproducible protocol for isolation of intracerebral arterioles (a group of blood vessels encompassing parenchymal arterioles, penetrating arterioles and pre-capillary arterioles) from mice, to be used in pressure myography, immunofluorescence, biochemistry, and molecular studies.
FAQs
Maintaining a patent cannula keeps access open for the intended intervention or measurement. If the pathway becomes obstructed, fluid delivery, drainage, and physiological monitoring may no longer proceed consistently. In experimental work, preserving patency supports dependable exposure, sampling, and measurements across the study while reducing a source of procedural variability.
Surgical cannulation technique depends on coordinating careful dissection with controlled tube placement and securement. Dissection creates access while limiting tissue injury, and securement helps keep the cannula positioned during subsequent delivery, drainage, or monitoring. Together, these steps help reduce bleeding, obstruction, and disruption that could compromise the experiment.
Sterile handling helps limit microbial contamination introduced during access and manipulation. In immunology and infection research, unintended contamination can complicate interpretation of host responses or disease progression. Maintaining sterile conditions therefore helps distinguish the effects of the planned experimental exposure from responses associated with unwanted microbial contamination.
The access site should be selected according to the function required in the experiment. A vessel may support delivery or sampling related to circulation, whereas a body cavity or organ conduit may provide access suited to other forms of fluid handling or monitoring. Matching site and purpose helps maintain a useful, interpretable pathway.
A basic workflow progresses from careful tissue dissection to hollow-tube insertion, followed by securement and maintenance of a patent pathway. Sterile handling is incorporated to limit microbial contamination, while attention to bleeding, obstruction, and tissue injury protects access quality. This sequence provides the controlled route needed for later intervention or measurement.
In immunology and infection research, cannulation can support controlled infusion of cells, pathogens, drugs, or fluids and permit serial sampling. It also enables measurement of host responses over an experiment. These capabilities improve consistency and help investigators examine inflammation, immunity, circulation, and disease progression under controlled conditions.