3d-last Technique
3d-last Technique - Related Videos
05:18
Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique
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Cited by 21 •
2016
Eliminating specific cells without damaging other cells is extremely difficult, especially in established tissue, yet there is an urgent need for a cell elimination method in the tissue engineering field. Here, we present a method for specific cell elimination from a mixed 3D cell culture using near infrared photoimmunotherapy (NIR-PIT).
05:29
3D DNA FISH: A Technique to Locate a Specific Gene on a Chromosome
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2023
Circulating tumor cells (CTC) isolation and characterization from the blood of cancer patients is a type of non-invasive liquid biopsy and associated with relapse and disease progression. This video describes the technique of DNA fluorescence-in-situ-hybridization (DNA FISH) on CTCs captured on a medical wire, to study a specific gene of interest.
11:34
High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
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Cited by 9 •
2013
This video describes the fundamentals of digital fringe projection techniques, which provide dense 3D measurements of dynamically changing surfaces. It also demonstrates the design and operation of a high-speed binary defocusing system based on these techniques.
03:53
Laser Microdissection: A Technique to Isolate Invading Colorectal Cancer cells from a 3D Organotypic Culture Model
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2023
The video describes the laser dissection of the 3D organotypic co-culture model with micron-level precision. In 3D organotypic models, stromal constituents such as fibroblasts are 3-dimensionally co-cultured with cancer epithelial cells. The 3D model serves as an experimental tool that enables invasion and cancer-stroma interactions to be studied in near-physiological conditions.
03:33
Hanging Drop Method: A Technique to Generate 3D Melanoma Spheroids
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2023
This video describes the technique of generating three-dimensional spheroids via the hanging drop method using melanoma cells. The generated in vitro three-dimensional (3D) organotypic melanoma spheroid model can portray the in vivo architecture of malignant melanoma and may give insights into intra-tumoral...
FAQs
The process typically starts with imaging or surface-scan information, which is converted into a digital three-dimensional reconstruction. Clinicians can then visualize the anatomy, take measurements, and modify the representation for a specific task. This workflow connects diagnostic data with practical analysis, allowing the same patient-specific information to support planning, simulation, design, or communication.
A three-dimensional representation shows individual anatomical relationships in a form that can be visualized and measured from multiple perspectives. That added spatial context may help clinicians understand complex anatomy and recognize technical considerations that are less apparent in two-dimensional views. The result is a more practical basis for decision-making, discussion, and preparation before an intervention.
Measurement allows the reconstructed anatomy to be examined quantitatively, while digital modification enables the model to be adapted for analysis, planning, or design. These functions make the model more than a visual display: they turn patient-specific information into a working representation that can support customized clinical aids, procedure preparation, and evaluation of possible approaches.
After digital reconstruction and modification, the model can be manufactured as a physical representation or used to guide the design of a clinical aid. This connection helps translate anatomical information into something clinicians can inspect, discuss, or use during preparation. It is especially relevant when patient-specific geometry matters for customized implants, orthoses, or procedural planning.
A typical workflow obtains imaging or surface-scan data, creates a digital anatomical reconstruction, and then visualizes or measures the result. Depending on the clinical objective, the model may be modified, used for procedure simulation, or manufactured as a physical model or aid. The final format therefore depends on whether the goal is analysis, planning, education, or intervention.
Clinicians may use it before an operation or other intervention when understanding patient-specific anatomy and anticipating technical challenges are important. The reconstructed model can support review, measurement, and simulation before the procedure takes place. By linking diagnostic information with preparation, it can help organize decisions and provide a clearer basis for discussing the planned approach.
A three-dimensional patient-specific model can make complex anatomy easier to visualize than conventional two-dimensional views alone. In education, it provides a concrete representation for studying anatomical relationships and planning procedures. In clinical communication, the model can help teams discuss findings, possible interventions, and technical issues using a shared visual reference.
The technique can support the design of customized implants or orthoses, as well as physical models used for analysis, planning, or education. These applications rely on representing the individual patient's anatomy rather than using only generalized forms. Its broader value is the ability to carry patient-specific diagnostic information into practical clinical decisions and intervention preparation.