Dura Intact Visualization

Dura intact visualization is the observation of the dura mater while this protective membrane remains undisturbed, an approach that supports anatomical assessment without opening the cranial coverings. It relies on surgical exposure, controlled illumination, and visual inspection of the intact dural surface to identify its position, contours, and relationship to surrounding structures. In medicine, this perspective can guide operative planning, anatomical teaching, and documentation of neurosurgical procedures while minimizing unnecessary manipulation of the dura. It also provides a reference for comparing normal anatomy with pathological changes and for developing techniques that preserve protective tissues during intervention.

Dura Intact Visualization - Related Videos

Research

JoVE EoE - Neuroimaging

Investigating the Origins of Sensory Neurons in the Dura Mater Using Fluorescence Microscopy

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2025

Source: Wang, J., et al. Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing. J. Vis. Exp. (2021). This video demonstrates a method to trace the origins of sensory neurons in the dura mater using fluorescence microscopy. A retrograde tracer is introduced into the exposed dura mater of an anesthetized rat. The tracer is taken up by sensory neuronal axons and travels retrogradely to their cell bodies...

Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

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Cited by 3 •

2021

Here we present a protocol to visualize spatial correlation of calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers and blood vessels in the cranial dura mater using immunofluorescence and fluorescent histochemistry with CGRP and phalloidin, respectively. In addition, the origin of these nerve fibers was retrograde traced with a fluorescent neural tracer.

Larval Fillet Preparation: A Method to Visualize Intact Sensory Neurons and Associated Epidermal Cells

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2023

This video describes how to prepare larval fillets used to visualize sensory dendritic arborization (da) neurons and their associated epidermal cells. The featured protocol shows in detail how to remove muscle tissue from the body wall while preserving the cells' morphology, which improves immunostaining and visualization of the otherwise obscured da neurons and epidermal cells.

Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets

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Cited by 12 •

2017

We describe a protocol for visualization of insulin exocytosis in intact islets using pHluorin, a pH-sensitive green fluorescent protein. Isolated islets are infected with adenovirus encoding pHluorin coupled to the vesicle cargo neuropeptide Y. This allows for the detection of insulin granule fusion events by confocal microscopy.

Single Cell Electroporation in vivo within the Intact Developing Brain

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Cited by 26 •

2008

Single-cell electroporation (SCE) is a specialized technique allowing delivery of DNA or other macromolecules into individual cells within intact tissue, including in vivo preparations. Here we detail the procedure for SCE of a fluorescent dye or plasmid DNA into neurons within the intact brain of the Xenopus laevis tadpole.

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