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Chapters
- 00:00Title
- 00:52Introduction
- 02:13Fabrication and Loading of Micropipettes
- 04:02Single-Cell Electroporation Technique
- 09:16Screening for Successfully Electroporated Cells
- 10:16In Vivo 2-Photon Imaging of Neurons Fluorescently Labeled by Single-Cell Electroporation
- 12:46Conclusion
Single-cell electroporation (SCE) is a specialized technique allowing delivery of DNA or other macromolecules into individual cells within intact tissue, including in vivo preparations. Here we detail the procedure for SCE of a fluorescent dye or plasmid DNA into neurons within the intact brain of the Xenopus laevis tadpole.
Tags
Single-cell ElectroporationIn VivoIntact Developing BrainDNA DeliveryMacromoleculesIndividual CellsExperimental ManipulationsCell-autonomous EffectsGlobal TreatmentsIn Vivo Imaging TechniquesFluorescent MarkersCellular MorphologyCell GrowthIntracellular EventsXenopus Laevis TadpolesVideo ArticleProcedure For SCEFluorescent DyePlasmid DNANeuronsAlbino Xenopus TadpoleYield OptimizationTwo-photon Fluorescence Imaging
