Dynamic Volume Loading Assessment
Dynamic Volume Loading Assessment - Related Videos
06:16
Rat Heterotopic Abdominal Heart/Single-lung Transplantation in a Volume-loaded Configuration
0 Views •
2015
We describe a novel technique for heterotopic abdominal heart-lung transplantation (HAHLT) in rats. The transplant configuration results in a partially loaded graft circulation, allowing direct functional assessment. This model may be employed for acute or chronic studies of function and immunologic status of the transplanted graft.
04:29
Assessing Pseudomonas syringae Infection Load in Arabidopsis thaliana Leaves
0 Views •
2026
Source: Liu, X., et al. Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem. J. Vis. Exp. (2015).This video demonstrates a method to assess the bacterial load in Arabidopsis thaliana leaves infected with Pseudomonas syringae. Leaf discs from infected leaves are homogenized, serially diluted, and plated onto nutrient-rich agar to promote bacterial colony formation. Colony counts are then used to...
06:14
Assessment of Mitochondrial Fission/Fusion Dynamics in Kidney Proximal Tubular Cells
0 Views •
2025
This article describes the use of confocal microscopy and ImageJ to assess mitochondrial fission/fusion dynamics via Dendra2 photo-switching fluorescence.
02:29
Isolation and Cultivation of Bacteria from Zebrafish Embryos to Assess Infection Load
0 Views •
2026
Source: Bernut, A., et al., Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos. J. Vis. Exp. (2015).This video demonstrates a protocol for isolating and quantifying Mycobacterium abscessus from an infected zebrafish embryo. The procedure involves embryo euthanasia, tissue lysis, and homogenization to release the bacteria, followed by centrifugation and resuspension in a surfactant buffer. Finally, the bacterial suspension is serially diluted, plated...
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
0 Views •
Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
FAQs
Static measures may not show whether additional circulating volume will improve cardiac performance. Dynamic Volume Loading Assessment instead examines the cardiovascular response to a controlled, temporary change in preload. This response-based approach provides information about likely benefit from fluid, supporting a more individualized decision than relying on blood pressure or central venous pressure in isolation.
Stroke volume and cardiac output show whether the heart increases forward flow when preload temporarily rises. An observed change indicates how cardiac performance responds under the tested condition, rather than merely describing the patient’s pressure or filling status. Monitoring these variables therefore links the assessment directly to the potential hemodynamic effect of intravenous fluid.
The approach separates two clinically important questions: whether fluid is likely to increase cardiac output and whether giving more fluid may be poorly tolerated. A patient may not demonstrate a useful flow response, making additional administration less attractive. This distinction helps clinicians avoid treating circulatory instability with fluid solely because instability is present.
Clinicians commonly use passive leg raising or a small fluid challenge to alter preload during the assessment. The cardiovascular response is then observed through stroke volume or cardiac output monitoring. These alternatives provide a structured test of hemodynamic response while keeping the evaluation focused on whether increased preload produces a meaningful improvement in flow.
First, clinicians assess the patient’s hemodynamic state and select a temporary preload intervention, such as passive leg raising or a small fluid challenge. They then monitor stroke volume or cardiac output during the response. The resulting change informs whether further intravenous fluid is likely to improve circulation, rather than relying only on static measurements.
It is particularly relevant for patients with circulatory instability when clinicians must decide whether intravenous fluid is appropriate. By estimating the likelihood of increased cardiac output, the assessment supports individualized therapy and may reduce unnecessary fluid administration. This matters because excessive or ineffective fluid treatment can contribute to associated complications and fluid intolerance.