Epididymal Adipose Tissue
Epididymal Adipose Tissue - Related Videos
06:17
Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues
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Cited by 1 •
2020
We present a simple method to isolate highly viable adipose progenitor cells from mouse epididymal fat pads using fluorescence activated cell sorting.
06:39
Fat-Covered Islet Transplantation Using Epididymal White Adipose Tissue
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Cited by 3 •
2021
This fat-covered islet transplantation method is suitable for the detection of engrafted islets in the intraperitoneal cavity. Notably, it does not require the use of biobinding agents or suturing.
07:09
Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering
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Cited by 58 •
2017
We present a protocol to isolate adipose tissue-derived microvascular fragments that represent promising vascularization units. They can be rapidly isolated, do not require in vitro processing and, thus, may be used for one-step prevascularization in different fields of tissue engineering.
04:53
Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue
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2024
The adipose tissue is an excellent source of mesenchymal stem cells. Here, we bring the step-by-step extraction, cultivation, and characterization of adipose tissue-derived stem cells (ADSCs) from Swiss mice epididymal adipose tissue.
07:09
Isolation of Adipose Tissue Immune Cells
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Cited by 75 •
2013
Adipose tissue (AT) is a site of intense immune cell activation and interaction. Almost all cells of the immune system are present in AT and their ratios are altered by obesity. Proper isolation, quantification, and characterization of AT immune cell populations are critical for understanding their role in immunometabolic disease.
FAQs
Insulin influences how adipocytes in this depot handle triglycerides, shifting the balance between lipid storage and mobilization. Energy demand can promote release of stored fatty acids, whereas insulin signaling supports retention of energy within triglycerides. Studying these responses helps researchers evaluate altered fat handling in metabolic disease models.
These secreted factors provide potential communication routes between adipose tissue, nearby reproductive structures, and distant organs. Adipokines can reflect adipose signaling, free fatty acids can indicate lipid mobilization, and inflammatory mediators can signal tissue stress. Measuring their changes helps connect local adipose activity with systemic metabolic and reproductive outcomes.
Changes in adipocyte lipid handling and tissue-derived signals may coincide with both metabolic abnormalities and altered reproductive function. Because the depot lies near the epididymis, its biology offers a way to examine how adipose-related inflammation, fatty-acid release, or adipokine signaling may relate to sperm-supporting tissues while also reflecting whole-body metabolic status.
Researchers analyze this depot by assessing features such as triglyceride storage and mobilization, adipokine production, free-fatty-acid release, and inflammatory mediator changes. They can then compare these findings with measures of obesity, insulin resistance, inflammation, reproductive function, or treatment response. This integrated approach links tissue-level changes with broader experimental outcomes.
The depot is particularly useful when an animal study addresses obesity, insulin resistance, inflammation, reproductive function, or the effects of a drug. Its analysis can show whether an intervention changes adipose lipid behavior or signaling and whether those changes accompany improved or worsened metabolic and reproductive findings.
Findings from the depot can help interpret whether disease or treatment is associated with altered triglyceride handling, adipose-derived signaling, fatty-acid release, or inflammatory activity. Researchers can relate those patterns to whole-body metabolism and reproductive outcomes, providing context for how fat distribution and tissue behavior contribute to medical conditions.