Follicular Viability
Follicular Viability - Related Videos
06:40
Collection of Human Follicular Fluid, Follicle Somatic Cells, and Immature Oocytes from Individuals Undergoing In Vitro Fertilization
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2025
This protocol outlines standardized methods to isolate and process follicular fluid, somatic cells, and immature oocytes from IVF procedures, enabling high-quality molecular and cellular analyses. These approaches support translational research into fertility, reproductive aging, and ovarian dysfunction using materials typically discarded in clinical care.
12:03
Viability Assays for Cells in Culture
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Cited by 39 •
2014
Therapeutic compounds are often first examined in vitro with viability assays. Blind cell counts by a human observer can be highly sensitive to small changes in cell number but do not assess function. Computerized viability assays, as described here, can assess both structure and function in an objective manner.
05:47
Counting and Determining the Viability of Cultured Cells
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Cited by 19 •
2008
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.
07:24
Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments
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Cited by 5 •
2016
Optimized procedures for the isolation of single follicles, cytoplasmic RNA microinjections, the removal of surrounding cell layers, and protein expression in Xenopus oocytes are described. In addition, a simple method for fast solution changes in electrophysiological experiments with ligand-gated ion channels is presented.
02:38
Fluorescence-Based Viability Assessment of Oral Anaerobic Bacteria
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2026
Source: Calatayud Arroyo, M., et al. Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface. J. Vis. Exp. (2018)The video demonstrates a method to evaluate the viability of anaerobic oral bacteria using fluorescent dyes and flow cytometry. It begins with culturing bacteria in oxygen-free broth, followed by resuspending a single colony from a blood agar plate. After incubation and dilution, the culture is stained with membrane-permeable and...
FAQs
The oocyte, granulosa cells, and theca cells must function together rather than being assessed as isolated structures. Their interactions support nutrient exchange, hormone signaling, and the local conditions needed for follicle survival. Damage affecting one cell population can therefore influence the developmental support available to the oocyte, making coordinated cellular integrity an important consideration in medical evaluation.
Nutrient exchange helps sustain the metabolic needs of the follicle, while hormone signaling coordinates activity among its cellular components. These processes are interconnected: structural preservation alone may not demonstrate that a follicle remains functional. Evaluating both cellular condition and activity provides a stronger indication of whether the follicle can continue supporting oocyte development after treatment or manipulation.
Morphology describes the follicle’s visible structural condition, whereas viability also considers cellular integrity and functional activity. A follicle may appear structurally intact yet require further evaluation to determine whether its cells remain metabolically active and responsive after culture, cryopreservation, or medical exposure. Combining these measures helps distinguish preserved appearance from preserved biological function.
Assessment can combine examination of follicle morphology, cellular integrity, and functional activity after a defined laboratory condition such as culture or cryopreservation. This approach allows investigators to compare whether follicles remain intact and active following handling. The resulting observations can identify conditions that preserve follicular function or reveal impairment associated with a treatment or experimental exposure.
After cryopreservation or culture, investigators can examine whether follicles retain appropriate morphology, cellular integrity, and functional activity. These measures are considered together because structural appearance alone may not show whether the follicle remains metabolically active or capable of supporting oocyte development. Such evaluations help determine how well a preservation or culture condition maintains follicular function.
In medicine, viability assessments help determine whether follicles remain suitable for strategies involving fertility preservation, ovarian tissue transplantation, or assisted reproduction. They also support reproductive-toxicity studies by showing whether a treatment protects or impairs follicular function. The information connects laboratory observations with the broader question of whether ovarian material retains functional reproductive potential after intervention.