Counting and Determining the Viability of Cultured Cells

Determining the number of cells and culture is important in the standardization of culture conditions and when performing experiments where precise cell numbers are needed. Here we will show you how to count cells using a hemo cytometer, and calculate the concentration of cells present in suspension. We will also show you how to determine the number of viable cells present in your culture with the Trian Blue Viability Assay.

Hi, I'm Ricky here at the Dr.LAN's Laboratory at Molecular Pathology Laboratories Network in East Tennessee. Today I'm going to show you how to count cells and suspension and how to determine their viability. In order to prepare the hemo cytometer for cell counting, you first want to clean its surface and the accompanying cover slip with 70%ethanol.