Intratumoral Injection
Intratumoral Injection - Related Videos
04:00
Modelling Intratumoral Treatment with miRNA Inhibitors: A Method to Analyze the Effect of miRNA Inhibitors on Tumor Growth in Murine Model
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2023
This video demonstrates the in vivo delivery of miRNA inhibitors as therapeutic agents against tumor growth in orthotopic mouse models. The intratumoral administration of synthetic miRNA inhibitors or antagomiRs allows them to bind and inhibit endogenous miRNAs, thereby causing mRNA upregulation and suppression of tumor growth.
09:23
Inducing Targeted Mild Hyperthermia in Murine Tumor Models through Photothermal Conversion of Near-infrared Light by Intratumoral Gold Nanorods
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2025
This protocol presents techniques and methodology necessary for targeted hyperthermic therapy in solid tumor models. The approach leverages the photothermal conversion of near-infrared light by intratumorally injected gold nanorods to induce localized heating within the tumor microenvironment.
04:04
Endobronchial Ultrasound-guided Intratumoral Injection of Cisplatin for the Treatment of Isolated Mediastinal Recurrence of Lung Cancer
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Cited by 13 •
2017
The management of isolated recurrent lung cancer in a previously-irradiated field is challenging. Here, we describe an endobronchial ultrasound (EBUS)-guided cisplatin injection for the management of patients with localized lung cancer recurrence in a previously-radiated field.
07:31
Intradermal Tumor Implantation as a Robust Model for Murine Cancer Intratumoral Immunotherapy Studies
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2026
With increasing interest in intratumoral immunotherapy, selecting tumor models that are accessible and practical is essential. Subcutaneous tumors are easy to establish but difficult to visualize and manipulate. In this protocol, we describe methods for generating intradermal tumors that provide improved visibility and accessibility.
08:28
Zebrafish Brain Ventricle Injection
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Cited by 72 •
2009
After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.
FAQs
Local delivery changes where treatment is concentrated, while the substance’s own activity determines what happens inside the tumor. After deposition, the agent can spread through nearby tumor tissue, damage cancer cells, modify the tumor microenvironment, or stimulate an immune response. These distinct effects explain why intratumoral injection can support different therapeutic strategies rather than one uniform mechanism.
The tumor microenvironment is an important target beyond the cancer cells themselves. An injected agent may alter conditions within the tumor and promote an immune response, potentially making the lesion a site of therapeutic activity. This matters in medicine because treatment effects can arise from both direct action on tumor tissue and biologic changes surrounding those cells.
Compared with administering treatment without direct placement in the tumor, this approach aims to concentrate the substance at the disease site and potentially reduce exposure of healthy tissues. The benefit depends on local diffusion and the agent’s effects in tumor tissue. It is therefore especially relevant when a localized effect is a central goal of treatment or investigation.
Combination regimens can use intratumoral injection as one component of a broader treatment strategy. Delivering an investigational therapy into a tumor allows researchers to examine how local treatment contributes to the overall response when paired with other approaches. This design is relevant to immunotherapy studies and therapy development, where direct tumor effects and immune effects may both matter.
The procedure centers on placing a needle within the solid tumor, with imaging used when appropriate to guide placement. Clinicians then deposit the selected therapeutic substance into the tumor tissue. The key procedural objective is accurate local delivery, because the deposition site influences subsequent diffusion and the ability to evaluate the treated lesion.
The delivered material may be a drug, biological agent, or investigational therapy, and the choice determines the expected biologic effect. Some substances may act directly on cancer cells, whereas others may alter the tumor microenvironment or stimulate immunity. This range allows medicine and clinical research to evaluate different mechanisms while using the same local delivery route.
Use is concentrated in selected treatments and clinical research rather than presented as a universal approach for every tumor. Investigators may study intratumoral injection in immunotherapy trials or combination regimens, while clinicians may consider it when localized treatment is appropriate. Its role is therefore both therapeutic and developmental: it can deliver a candidate therapy and support evaluation of its effects.
Because treatment is deposited in a defined tumor, researchers can directly assess the response of that lesion during therapy development. Evaluation can focus on how the tumor tissue reacts to the injected substance, including evidence of cancer-cell damage, microenvironmental change, or immune activity. This local readout helps connect the delivered therapy with its observed tumor response.