Lymphocyte Count
Lymphocyte Count - Related Videos
10:19
Using a Hemacytometer to Count Cells
0 Views •
2023
Many biomedical experiments require manipulation of a known quantity of cells, in order to achieve accurate, reproducible, and statistically-relevant data. Therefore, learning how to count cells is a particularly essential technique for any successful biomedical scientist. The most common way to count cells is by using a hemacytometer - an instrument that bears two laser-etched grids, which aid in the enumeration of an aliquot cells under a simple light microscope. This data can then be used to...
06:02
Isolation of Lymphocytes from a Mouse Peyer's Patch
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2025
In this video, we demonstrate the isolation of lymphocytes from a harvested mouse Peyer's patch. The isolated lymphocytes can be used for further analysis.
03:23
An In Vitro Technique to Stimulate Lymphocytes via Pathogenic Bacteria
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2025
This video demonstrates an in vitro technique to stimulate lymphocytes via non-typeable Haemophilus influenzae (NTHi) and to assess the immune response. Incubating isolated peripheral blood mononuclear cells (PBMCs) with NTHi causes the bacterial cells to stimulate T lymphocytes, leading to cytokine production. Upon inhibiting the secretion of cytokines, immunofluorescence staining is performed to detect the accumulated intracellular cytokines in stimulated T lymphocytes.
05:47
Counting and Determining the Viability of Cultured Cells
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Cited by 19 •
2008
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.
12:59
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
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Cited by 33 •
2013
With effector functions distinct from other T cell subsets, Th17 cells have been centrally implicated in inflammatory autoimmunity. This in vitro Th17 differentiation protocol provides a means to determine whether naïve CD4+ T lymphocytes can differentiate into Th17 cells, and to further examine their role in autoimmunity and host response.
FAQs
The differential separates lymphocytes from neutrophils, monocytes, eosinophils, and basophils within the circulating white blood cell population. This distinction allows the reported lymphocyte count to reflect one specific white blood cell group rather than total white blood cells. The result therefore provides more focused information about immune system activity and supports comparison with other components of the blood count.
An unusually high or low result is not interpreted as evidence of one condition by itself. The overview identifies infections, immune disorders, inflammatory conditions, and some blood cancers as possible clinical contexts. Symptoms, age, medications, and other laboratory findings help determine which explanation is more consistent, preventing the isolated number from being treated as a diagnosis.
These accompanying factors place the laboratory result in its clinical context. The same measured count may have different significance depending on a person's age, current symptoms, medications, and findings from other tests. Considering them together improves interpretation and helps clinicians evaluate whether an abnormal result supports investigation of infection, immune dysfunction, inflammation, or a blood cancer.
Repeated measurements add a time-based perspective that a single result cannot provide. Comparing counts can help monitor disease progression or assess response to treatment, particularly when the clinical team is following an abnormal finding. The trend remains part of a broader evaluation, because interpretation still depends on symptoms and other laboratory information rather than on the count alone.
It is usually determined from a blood sample analyzed as part of a complete blood count with differential. The analysis examines circulating white blood cells and distinguishes lymphocytes from the other listed white blood cell types. Including the measurement within this broader test provides related blood-cell information that can be considered together during medical evaluation.
Clinicians may review it when evaluating possible infections, immune disorders, inflammatory conditions, or some blood cancers. It can also be useful when monitoring an established disease over time or assessing response to treatment. Because the count is commonly included in a complete blood count with differential, it contributes to a broader laboratory assessment rather than serving as a standalone test.
An abnormal count can signal that additional clinical interpretation may be needed, but it does not establish a specific cause on its own. High or low values can occur in several medical contexts, so clinicians compare them with symptoms, age, medications, and other laboratory findings. This combined assessment determines the result's relevance to diagnosis, monitoring, or treatment evaluation.