Murine Pneumonia Model
Murine Pneumonia Model - Related Videos
09:17
A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii
0 Views •
Cited by 17 •
2017
A method for establishing lung infections in immunocompetent rodents is described. With proficiency, this method can be performed quickly and easily to induce stable infection with many isolates of S. pneumoniae, H. influenzae, P. aeruginosa, K. pneumoniae and A. baumannii.
04:46
Intrabronchial Instillation of Streptococcus pneumoniae in Immunocompetent Rodent Models
0 Views •
2025
This video demonstrates the establishment of lung infection in an immunocompetent rodent model via intubation and installation of bacterial suspensions into lung lobes. This model enables antibacterial efficacy studies in immunocompetent animals.
00:13
A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
0 Views •
Cited by 2 •
2019
Here, we present methods to improve secondary bacterial pneumonia studies by providing a non-invasive route of instillation into the lower respiratory tract followed by pathogen recovery and transcript analysis. These procedures are reproducible and can be performed without specialized equipment such as cannulas, guide wires, or fiber optic cables.
04:32
Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia
0 Views •
Cited by 35 •
2018
Infectious pneumonia is among the most common infections in human. An appropriate in vivo model is critical for understanding disease pathogenesis and testing the efficacy of novel therapeutics. With this murine oropharyngeal aspiration pneumonia model, one can examine the pathogenesis and new treatments against these deadly infections.
03:17
A Mouse Model to Study the Pathogenic Transition of Streptococcus pneumoniae Following Viral Co-Infection
0 Views •
2026
Source: Lenhard, A. et al. A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness. J. Vis. Exp. (2022)This video demonstrates a mouse co-infection model in which the influenza virus triggers the transition of Streptococcus pneumoniae from a colonizer to a pathogen. It outlines the steps involved in bacterial colonization, viral infection, and disease progression.
FAQs
The inoculation route can shape how infection is established in the respiratory tract. Intranasal delivery introduces the pathogen through the nose, whereas intratracheal delivery places it directly into the trachea. Comparing these routes helps investigators examine how experimental conditions influence airway infection and the resulting pattern of host responses and lung injury.
Inflammatory cell recruitment and cytokine production connect pathogen exposure to tissue-level disease. As immune cells enter the lungs and signaling molecules accumulate, investigators can relate immune activation to changes in lung tissue. Measuring these linked responses helps distinguish pathogen-driven effects from host-mediated injury and clarifies mechanisms underlying pneumonia severity.
Mice with different genetic backgrounds can respond differently to the same experimental infection. Comparing those groups allows researchers to examine host susceptibility rather than treating disease as uniform across animals. This design can reveal how inherited biological differences influence pathogen burden, immune responses, lung pathology, or survival under otherwise comparable conditions.
After a respiratory pathogen is introduced by an intranasal or intratracheal route, the infection is allowed to establish in the airways. Investigators then assess pathogen burden, immune responses, lung pathology, and survival. These coordinated measurements connect the experimental exposure with biological changes and clinically relevant outcomes in the model.
The model supports testing of antimicrobial therapies, anti-inflammatory therapies, and vaccines, while also enabling investigation of pneumonia pathogenesis. Researchers can ask whether an intervention changes infection, host inflammation, tissue damage, or survival. It therefore links mechanistic studies of disease with evaluation of potential treatment and prevention strategies.
Pathogen burden, immune responses, lung pathology, and survival represent complementary endpoints. Examining them together helps determine whether experimental conditions or interventions are associated with changes in infection, host defense, tissue effects, or overall outcome. This multidimensional assessment is especially useful when comparing disease mechanisms across genetic backgrounds or controlled experimental conditions.