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A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii

A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii

DOI:
10.3791/55068-v

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09:17 min

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January 02, 2017

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, , , , ,
1Antibacterial Discovery Performance Unit,GlaxoSmithKline

Chapters

  • 00:05Title
  • 01:13Prepare Saline Bacterial Suspensions and Agar-based Inoculum
  • 02:20Prepare Intubation Device, Intubate and Inoculate Rats
  • 05:47Prepare Intubation Device, Intubate, and Inoculate Mice
  • 07:10Results: Evaluation of Antibacterial Efficacy in Infected Rats and Mice
  • 08:37Conclusion

Summary

Automatic Translation

Automatic Translation

A method for establishing lung infections in immunocompetent rodents is described. With proficiency, this method can be performed quickly and easily to induce stable infection with many isolates of S. pneumoniae, H. influenzae, P. aeruginosa, K. pneumoniae and A. baumannii.

Tags

Keywords: Pneumonia ModelImmunocompetent RodentsAntibacterial EfficacyS. PneumoniaeH. InfluenzaeK. PneumoniaeP. AeruginosaA. BaumanniiIntrabronchial InfectionBacterial PneumoniaImmunocompetent ModelRobust And Reproducible Lung InfectionIntubationTracheaBacterial SuspensionAgar PlatesSalineSerial DilutionAgarWater BathIntubation Device

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