Alpha4yfp Knock-in Mice
Alpha4yfp Knock-in Mice - Related Videos
08:04
Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.
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Cited by 7 •
2017
We describe a HPLC-based method for the determination of N-acetylneuraminic acid and N-glycolylenuraminic acid in mouse liver and milk.
09:07
Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
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Cited by 21 •
2015
We describe a method for the generation of in vitro derived mast cells, their engraftment into mast cell-deficient mice, and the analysis of the phenotype, numbers and distribution of engrafted mast cells at different anatomical sites. This protocol can be used to assess the functions of mast cells in vivo.
08:47
Spectral Confocal Imaging of Fluorescently tagged Nicotinic Receptors in Knock-in Mice with Chronic Nicotine Administration
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Cited by 12 •
2012
We have developed a novel technique of quantifying nicotinic acetylcholine receptor changes within subcellular regions of specific subtypes of CNS neurons to better understand the mechanisms of nicotine addiction by using a combination of approaches including fluorescent protein tagging of the receptor using the knock-in approach and spectral confocal imaging.
08:48
Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice
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Cited by 13 •
2022
The present protocol describes zygote microinjection of CRISPR-Cas9 and donor DNA to efficiently produce gene cassette knock-in and floxed mice.
09:17
Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
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2025
Here, we describe a protocol for generating maternal mutant that couples a stable zpc:cas9 knock-in line with Tol2-mediated delivery of sgRNA expression cassettes.
FAQs
The endogenous gene placement makes the fluorescent signal informative about alpha4-containing receptor expression rather than an independently introduced marker. Because the YFP sequence is inserted into Chrna4, fluorescence remains linked to the receptor’s natural expression pattern. This allows investigators to examine where these receptors occur in neural tissue and interpret distribution changes in relation to cholinergic signaling.
Examining fluorescence at cellular and synaptic locations can distinguish broad receptor distribution from more specific cellular localization. Tracking the signal also supports analysis of receptor trafficking, meaning changes in receptor positioning within neural tissue. These observations help connect alpha4-containing receptor organization with synaptic organization and the operation of neuronal circuits.
Because the reporter is tied to the endogenous Chrna4 gene, researchers can compare receptor-associated fluorescence under different biological or experimental conditions. Patterns may be examined during development, in disease-related studies, or after pharmacological treatment. Such comparisons can show how alpha4-containing receptor expression or localization changes within neural tissue.
Researchers can use the mice by examining YFP signal in living neural tissue, then relating observed patterns to receptor distribution, cellular localization, or trafficking. The approach supports analysis of receptor-associated changes across neural structures and experimental conditions. This provides a visual platform for studying receptor organization in relation to cholinergic signaling.
In neuroscience, the model is especially relevant when the question concerns alpha4-containing receptors within cholinergic signaling. Fluorescent localization can be considered alongside synaptic organization, neuronal circuits, and behavior, allowing receptor patterns to be related to larger levels of neural function. This makes the mice useful for connecting molecular receptor placement with systems-level questions.
Researchers can use the model as a platform to examine whether receptor-associated fluorescence changes during disease or after pharmacological treatment. Such studies may assess altered expression, distribution, trafficking, or cellular localization in neural tissue. Linking these observations to cholinergic signaling provides a way to investigate how experimental conditions affect receptor organization and related neural processes.