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Analyzing the Functions of Mast Cells In Vivo Using ‘Mast Cell Knock-in‘ Mice
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Analyzing the Functions of Mast Cells In Vivo Using ‘Mast Cell Knock-in‘ Mice

Analyzing the Functions of Mast Cells In Vivo Using ‘Mast Cell Knock-in‘ Mice

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09:07 min

May 27, 2015

DOI:

09:07 min
May 27, 2015

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Summary

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We describe a method for the generation of in vitro derived mast cells, their engraftment into mast cell-deficient mice, and the analysis of the phenotype, numbers and distribution of engrafted mast cells at different anatomical sites. This protocol can be used to assess the functions of mast cells in vivo.

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