Dulbecco Pbs
Dulbecco Pbs - Related Videos
08:05
Mammosphere Assay Reveals Api5-Induced Stemness in Non-Tumorigenic Breast Epithelial Cell Lines
0 Views •
2026
This protocol describes the method for setting up the mammosphere formation assay to study Apoptosis Inhibitor 5 (Api5)- induced cancer stemness in non-tumorigenic breast epithelial cells. Lysates collected from primary generation mammospheres are analysed for different stemness markers to assess the induction of stem cell-like properties.
03:44
Developing a Triple Cell Culture Model of the Human Blood-Brain Barrier
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2025
This video demonstrates the co-culturing of human brain pericyte, astrocyte, and endothelial cells. Each cell layer is formed separately and then cultured together, creating a three-layered system that mimics the cellular arrangement of the blood-brain...
11:42
Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System
0 Views •
Cited by 4 •
2014
Using a pneumatic bioreactor, we demonstrate the assembly, operation, and performance of this single-use bioreactor system for the growth of mammalian cells.
08:03
Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
0 Views •
Cited by 14 •
2017
In this method, human primary muscle cells are cultured in vitro to obtain differentiated myotubes and glucose uptake rates are measured. We provide a detailed protocol to quantify rates in basal and insulin-stimulated states using radiolabeled [3H] 2-deoxy-D-Glucose.
02:25
Differentiating Embryoid Body Cells into Neural Progenitors Using Retinoic Acid
0 Views •
2025
The video demonstrates the method to induce stem cell differentiation into neural progenitors using retinoic acid and the hanging drop culture method.
FAQs
DPBS maintains sample conditions through two coordinated features: sodium chloride supports osmotic balance, while phosphate components help stabilize pH. This reduces physiological stress during handling, particularly when cells or tissue are temporarily outside nutrient-rich culture medium. Consistent buffering and salt composition can therefore make repeated laboratory manipulations more reproducible.
The presence or absence of calcium and magnesium is a formulation variable rather than a minor labeling difference. It allows investigators to match DPBS to the requirements of a particular rinse, wash, tissue preparation, or staining workflow. Selecting the appropriate version helps avoid introducing components that the experiment does not need.
Its defined salt and phosphate environment supports short-term maintenance of osmotic and pH conditions without adding constituents from culture medium. That makes DPBS useful when researchers need to rinse neural cultures, wash tissue, or prepare samples while limiting changes to the surrounding experimental conditions.
In a neural-culture or tissue workflow, DPBS can be applied as a rinse or wash before a downstream procedure. It can also support sample preparation for dissociation, immunostaining, or microscopy. The solution helps limit osmotic and pH stress, allowing the sample to enter the next step under more controlled conditions.
DPBS provides a non-nutrient, buffered salt environment while tissue or cultured cells are being prepared for dissociation. Because the formulation helps maintain osmotic balance and pH during handling, it can support more consistent preparation of material for subsequent neuroscience analyses, including work with neurons and glia.
Neural cultures, brain tissue, neurons, glia, and cell-based disease models can all require controlled washing or sample handling. DPBS fits these workflows for rinsing cultures, washing tissue, and supporting immunostaining or microscopy preparation. Its consistent composition is especially relevant when investigators compare samples, because variation in handling solution can affect reproducibility.