Fixation Device
Fixation Device - Related Videos
06:57
Instillation and Fixation Methods Useful in Mouse Lung Cancer Research
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Cited by 48 •
2015
The goal of this paper is to describe simple methods that will greatly aid in the setup and analysis of mouse lungs with lung cancer or other pathologies. We present 3 protocols to simply and reliably carry out lung instillations, fixation, and lung volume measurements.
08:53
Whole Animal Perfusion Fixation for Rodents
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Cited by 531 •
2012
Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.
08:29
Thermal Measurement Techniques in Analytical Microfluidic Devices
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Cited by 6 •
2015
Here, we present three protocols for thermal measurements in microfluidic devices.
05:58
Mouse Electroacupuncture Fixation Device Fabrication for Electroacupuncture Pretreatment in Diabetic Cardiomyopathy Mouse Model
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2025
Here, we present a protocol to establish a mouse model of diabetic cardiomyopathy and a mouse electroacupuncture fixation device for the use of electroacupuncture in mice, which can fix the mice more gently during treatment.
01:03
Fixation and Sectioning
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2023
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens. The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
FAQs
Effective fixation must hold tissue, an anatomical structure, or an implant firmly enough to limit disruptive movement while leaving sufficient access for surgery, recording, microscopy, or behavioral procedures. Excessive restraint can obstruct the target area or interfere with normal neural function. Researchers therefore select supports, clamps, frames, or holders according to the procedure and the structure being stabilized.
Uncontrolled movement can reduce the accuracy and reproducibility of neural measurements by changing the position of tissue, implants, instruments, or the experimental subject. Stabilization helps maintain a consistent relationship between the target and the measurement system. This is especially important when researchers perform stereotaxic surgery, electrophysiological recordings, microscopy, or behavioral experiments that depend on repeatable positioning.
Each application requires a different balance between stabilization and access. Stereotaxic surgery depends on consistent positioning for targeting specific brain regions, electrophysiological recording requires the tissue and instruments to remain aligned, and microscopy requires minimal movement that could disrupt imaging. A suitable device must support the relevant procedure without obstructing the tissue or experimental implant.
Placement should account for the location of the tissue or implant, the access needed for the planned procedure, and the risk of injury or interference with normal neural function. Researchers also need enough stability to protect delicate samples and instruments. These considerations determine where supports, clamps, frames, or holders can be positioned while preserving the intended measurement.
Researchers use these devices when movement could compromise a surgical procedure, measurement, image, or behavioral experiment. Applications include stabilizing tissue during stereotaxic surgery, maintaining an experimental implant during electrophysiological recording, and holding structures consistently for microscopy. The same principle also supports behavioral experiments when a controlled and repeatable position is necessary for meaningful observations.
Proper fixation can improve measurement accuracy and reproducibility by maintaining a consistent position throughout an experiment. It can also protect delicate samples and instruments and help researchers target specific brain regions more reliably. These benefits depend on appropriate device design and placement, because stabilization that causes injury or interferes with normal neural function can undermine the experimental outcome.