Glycerol Dapi
Glycerol Dapi - Related Videos
02:35
Long-Term Cryopreservation of Cyanobacterial Strains Using Glycerol or Dimethyl Sulfoxide
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2025
Source: Lea-Smith, D. J., et al. Generation of Marked and Markerless Mutants in Model Cyanobacterial Species. J. Vis. Exp. (2016)This video demonstrates the cryopreservation of cyanobacterial strains by combining concentrated cell suspensions with glycerol or DMSO and storing them at low temperatures, enabling long-term maintenance of viable cultures for photosynthesis and genetic studies.
03:32
Ethanol Fixation and DAPI Staining: A Method to Visualize DNA in C. elegans
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2023
Here, we describe a technique used to visualize genomic DNA in fixed, intact nematodes. The video includes an example protocol to count chromatin in unfertilized C. elegans oocytes.
13:02
Archimedes-Based Glycerol Displacement for Electrode Porosity Measurement in Lead-Acid Batteries
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2026
Here, we present a simple and low-cost glycerol displacement method based on Archimedes' principle for measuring lead-acid battery electrode porosities. The method demonstrates good reproducibility and is safer than traditional techniques, requiring low-hazard chemicals and equipment. Combining the technique with microstructural analysis by X-ray diffraction provides information on battery electrode properties and degradation.
08:03
Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells
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Cited by 9 •
2014
Synthesis and fabrication of electrospun long fibers spanning a larger deposit area via a newly designed collector from a novel biodegradable polymer named poly(glycerol-dodecanoate) (PGD) was reported. The fibers were able to support the growth of cells derived from mouse pluripotent stem cells.
03:23
Cell Death Screening Using DAPI Staining: A Method for Rapid Screening of IR Induced Clonogenic Cell Death in Cancerous Cell Lines
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2023
This video demonstrates the methodology to screen for major cell death profiles in ionizing radiation (IR) induced cancer cell lines using DAPI staining assay. The DAPI-stained nuclei of target cells undergoing cell death exhibit distinct morphologies and can be easily identified by fluorescence microscopy.
FAQs
The signal depends on DAPI binding to A-T-rich regions of double-stranded DNA after the dye enters fixed cells. Ultraviolet or near-ultraviolet excitation then produces blue fluorescence from labeled nuclei. Because the fluorescence reflects DNA-associated dye, the observed signal marks nuclear locations rather than directly reporting a particular neuronal protein or cellular process.
Glycerol serves as the specimen-preserving component of the mounting medium. Its contribution is distinct from DAPI's labeling function: DAPI supplies the fluorescent nuclear signal, whereas glycerol helps maintain the mounted sample for microscopy. This combination allows nuclear visualization to be performed on a preserved preparation rather than requiring the tissue or cells to remain unmounted.
Nuclear labeling supplies a positional reference for interpreting markers of neural structures or proteins. In brain tissue or cultured cells, the nuclei help relate a signal to the distribution and organization of cells, while the additional markers provide molecular or structural information. This complementary use supports quantitative analyses without treating the nuclear stain as a substitute for those markers.
The specimen must first be fixed so that DAPI can permeate the cells and bind their DNA. The preparation is then mounted in the glycerol-containing medium, and the mounted sample is examined by fluorescence microscopy using ultraviolet or near-ultraviolet excitation. This workflow preserves the sample while generating a blue nuclear reference for subsequent imaging.
In neuroscience, the preparation can support imaging of neuronal and glial nuclei in brain tissue as well as in cultured cells. The resulting nuclear map helps reveal how cells are distributed within a tissue or culture. When combined with neural markers, it can also provide anatomical context for fluorescence signals associated with structures or proteins.
Blue nuclear fluorescence can serve as a reference for assessing cell distribution and tissue organization. It also supports quantitative analyses performed alongside markers for neural structures or proteins, because nuclear positions provide a consistent cellular framework for comparing labeled features. Interpretation should focus on spatial organization and nuclear-associated measurements, not on assigning molecular identity from DAPI alone.