Paraffin Film Use
Paraffin Film Use - Related Videos
07:58
Electroplating of Thin Films
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2023
Source: Logan G. Kiefer, Andrew R. Falkowski, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Electroplating is a process that uses electric current to reduce dissolved metal cations so that they form a thin coating on an electrode. Other thin film deposition techniques include chemical vapor deposition (CVD), spin coating, dip coating, and sputter deposition among others. CVD uses a gas-phase precursor of the element to be...
08:30
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
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Cited by 53 •
2016
This modified extraction protocol improves RNA and DNA yields from more precisely targeted regions of interest in histopathologic tissue blocks.
11:27
Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
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Cited by 2 •
2008
We describe a reliable method for immunolocalization within the epidermis modified for both frozen and paraffin sections that we use very routinely in our laboratory.
07:28
Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles
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Cited by 42 •
2010
Identification of mechanisms underlying muscle damage is crucial. Here we present the histological technique for preparing paraffin-embedded and frozen sections of Drosophila thoracic muscles. This allows analysis of muscle morphology and localization of protein and other muscle cell components.
03:08
Embedding Adult Drosophila Heads in Paraffin: Preparing Tissue for Histology
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2023
This video describes how to embed Drosophila heads in paraffin using the collar method, a technique that prepares fly heads for serial sectioning. Paraffin sections are used for histological examinations of the brain's anatomy and for the assessment of neurodegeneration. The featured protocol demonstrates how multiple flies can be processed in one preparation, thereby increasing time-efficiency and minimizing experimental artifacts.
FAQs
Stretching the film before pressing it onto a vessel rim allows the material to conform closely to the container’s shape. This contact creates a water-resistant barrier, while the film’s flexibility helps it accommodate different rims and container formats. The resulting closure reduces direct exposure to surrounding air and helps protect contents during laboratory handling.
The film helps limit air exchange, moisture loss, and contamination by covering the vessel opening with a conforming barrier. These effects are important when contents must remain protected from surrounding laboratory conditions. By reducing exposure and evaporation, the seal supports more consistent handling of reagents, biological samples, and tissue-processing materials.
Maintaining stable volumes helps preserve consistency when researchers handle sensitive biological materials. Paraffin Film supports this objective by limiting moisture loss from sealed containers, which can otherwise alter the amount of liquid present. More consistent volumes can contribute to reliable cell culture, molecular assay, and specimen-handling workflows in neuroscience laboratories.
Application requires stretching the flexible film and pressing it into place over the container rim. The material conforms to the vessel as it is pressed, producing a water-resistant closure around the opening. After the container is no longer needed, the film can be removed without specialized tools, simplifying routine laboratory handling.
Neuroscience researchers can use the film with culture plates, sample tubes, reagent bottles, and tissue-processing containers. These formats support several workflow stages, including cell culture, molecular assays, and specimen storage. Selecting the film for these containers helps reduce environmental exposure while keeping routine sample and reagent handling consistent.
The film is useful when samples, reagents, or processed tissues need protection during temporary handling or storage. Its removable seal supports workflows in which containers may need to be accessed again without specialized opening equipment. In neuroscience laboratories, this can help maintain stable contents and reduce exposure-related variation across experimental procedures.