This content is Free Access.
Chapters
- 00:05Title
- 01:23Introduction
- 01:49Setting Up
- 02:33Fixing Flies in Collars
- 03:12Paraffin Sections of Drosophila Thoraxes
- 04:38Cryosections of Drosophila Thoraxes
- 05:39Lipid Detection in Drosophila Muscles
- 06:18Thoracic Muscles of Drosophila
- 07:08Conclusion
Identification of mechanisms underlying muscle damage is crucial. Here we present the histological technique for preparing paraffin-embedded and frozen sections of Drosophila thoracic muscles. This allows analysis of muscle morphology and localization of protein and other muscle cell components.
Tags
Paraffin-embedded SectionsFrozen SectionsDrosophila Adult MusclesMolecular CharacterizationMuscular DystrophiesMyopathiesMuscle DiseaseGenetic AnalysisMuscle SpecificationMuscle FormationMuscle FunctionModel SystemsMuscle PhysiologyMuscle DamageDrosophila Multi-fiber MusclesVertebrate Striated MusclesDystrophic Muscle MorphologyMuscular FunctionAgeing Adult FliesHistological TechniqueStainingProtein Detecting DyesCryosectionsImmunohistochemical DetectionMorphological DefectsExpression PatternMuscle/neuron-specific Proteins
