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Preparation of Aplysia Sensory-motor Neuronal Cell Cultures
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JoVE Journal Biologie
Preparation of Aplysia Sensory-motor Neuronal Cell Cultures

Preparation of Aplysia Sensory-motor Neuronal Cell Cultures

DOI:
10.3791/1355-v

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17:27 min

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June 08, 2009

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, ,
1Dept. of Psychiatry and Biobehavioral Sciences,University of California, Los Angeles, 2Dept. of Biological Chemistry,University of California, Los Angeles, 3Semel Institute for Neuroscience and Human Behavior,University of California, Los Angeles

Kapitel

  • 00:05Titel
  • 00:37Introduction
  • 01:22Preparation
  • 03:42Preparation of Hemolymph
  • 05:33Dissecting the Ganglia from Aplysia
  • 10:05Removing and Culturing Neurons from the Pleural Ganglia
  • 12:25Isolating Motor Neurons and Preparing Co-cultures with Sensory Neurons
  • 16:53Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Primary cultures of Aplysia sensory-motor neurons provide a model preparation for studying synapse formation and synaptic plasticity in vitro. This video demonstrates the identification and microdissection of sensory and motor neurons from Aplysia ganglia as well as the methods for establishing and maintaining sensory-motor neurons in culture.

Tags

Aplysia Sensory-motor Neuronal Cell CulturesMarine MolluskAplysia CalifornicaNeuronsGangliaCircuitryGill-withdrawal ReflexTactile StimulationSynaptic PlasticitySerotoninShort-term FacilitationIntermediate-term FacilitationLong-term FacilitationPeptide Transmitter FMRFamideSynaptic Weakening

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