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Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
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Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

Article DOI: 10.3791/1524-v • 12:18 min • October 28th, 2009
October 28th, 2009 •
1, 1, 1
1Stem Cell Research Unit, Medical University of Graz, Austria

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Summary
Automatic Translation

Endothelial colony forming progenitor cells (ECFCs) are a promising tool to study vascular homeostasis and repair.1,2 This paper introduces a novel animal-serum free method for isolation and expansion of ECFC from heparinised adult human peripheral blood with pooled human platelet lysate (pHPL) diminishing the risk of anti-bovine immunisation.

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Isolation Large Scale Expansion Adult Human Endothelial Colony Forming Progenitor Cells Recovery Strategy Heparinized Blood Peripheral Blood PHPL Bovine Serum Antigens Flow Cytometry Immunohistochemistry In Vitro Functionality Vascular Structures Matrigel Assay Subcutaneous Injection Mouse Model Perfused Vessels Cryopreservation Proliferation Genomic Stability Animal-protein Free Isolation
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