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Cancer Research

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Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts (Video) | JoVE
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Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts (Video) | JoVE

Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts from Fresh Tissue

Article DOI: 10.3791/200204-v • 05:38 min • May 26th, 2023
May 26th, 2023 •
*1,2,3,4, *5, 6, 6, 1,3, 6, 7, 6,8, 6,8, 3, 3, 2,9, 9, 10, 2,7, 11, 1,2, 1,2, 1,2,4, 3,12,13, 2,3,12,13, 3, 6, 1,2,3
1Molecular Epidemiology and Predictive Tumor Markers Group, Area 3, Ramón y Cajal Health Research Institute (IRYCIS), 2The Biomedical Research Network in Cancer (CIBERONC), 3Biobank and Biomodels Platform (PT20/0045), ISCIII research and development platforms in biomedicine and health sciences, BioBank Hospital Ramón y Cajal-IRYCIS, Spanish National Biobanks Network (ISCIII Biobank Register No. B.0000678), Ramón y Cajal Health Research Institute (IRYCIS), 4Faculty of Medicine, University of Alcalá de Henares, 5Institute of Tissue Medicine and Pathology, University of Bern, 6Department of Molecular Oncology, Cancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, 7Experimental Oncology, Molecular Oncology Program, Centro Nacional de Investigaciones Oncológicas (CNIO), 8Department of Surgical Oncology, National Cancer Institute, Slovak Medical University, 9Pancreatic and Biliopancreatic Surgery Unit, Hospital Universitario Ramón y Cajal, 10Department of Pathology, Hospital Universitario Ramón y Cajal, 11Department of Radiation Oncology, Hospital Universitario Ramón y Cajal, 12Department of Cancer, Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), 13Cancer Stem Cell and Fibroinflammatory Group, Chronic Diseases and Cancer, Area 3, IRYCIS
* These authors contributed equally

Tags

Cancer Research Número 195 Fibroblastos Modelos tumorales ex vivo Tejidos tumorales primarios Investigación traslacional del cáncer Sensibilidad y resistencia al tratamiento Interacciones célula-célula Microambiente tumoral Técnicas de cultivo Cócteles de factores de crecimiento Membrana basal biológica Recolección de muestras de tejido Biobanco y almacenamiento Capacidades de laboratorio SOP/protocolo Adenocarcinoma de páncreas Xenoinjertos derivados del paciente (PDX) Cultivo de tejidos Instalaciones de ratones
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