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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples

Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

Journal (Immunology and Infection)

Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro

Journal (Immunology and Infection)

Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro

Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples

Staining and Imaging to Visualize Neutrophil Extracellular Traps

Article DOI: 10.3791/200337-v • 04:01 min • August 18th, 2023

August 18th, 2023 •

Lavinia Schoenfeld¹, Birgit Appl¹, Laia Pagerols-Raluy¹, Annika Heuer³, Konrad Reinshagen¹, Michael Boettcher^1,2

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, University of Hamburg, ²Department of Pediatric Surgery, University Medical Center Mannheim, University of Heidelberg, ³Division of Spine Surgery, Department of Trauma and Orthopedic Surgery, University Medical Center Hamburg-Eppendorf (UKE)

Tags

Immunofluorescence Imaging Neutrophil Extracellular Traps NETs Bacterial Infection Traumatic Tissue Damage Autoimmune Diseases Sterile Inflammation Double-stranded DNA Filaments Histones Antimicrobial Proteins Pathogens Homeostatic Regulation Platelet Adhesion Coagulation Sepsis Autoimmune Disorders Therapeutic Intervention Electron Microscopy Staining Methods Antibodies Autofluorescence-reducing Agents Antigen Retrieval Methods Permeabilization

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