Encyclopedia of Experiments: Biology
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Acute Biliary Pancreatitis Model - ConceptTranscript
After checking the depth of anesthesia with a toe pinch, clean the abdominal area of an anesthetized mouse with a 5% povidone-iodine solution. Using a trimmer, remove hair between the chest and lower abdomen, and clean an approximately 2 square centimeter surgical area with 70% alcohol. Immobilize the animal on the surgical board and use scissors to cut 5 millimeters of the skin horizontally on the upper part of the abdomen and 1 centimeter below the xiphoid process. Repeat the cut on the peritoneum.
To perform the laparotomy with minimal exposure of the cavity, using a tractor pull the liver towards the head approximately 1 centimeter from the intestine and locate the region of the pancreas and the duodenum. Using forceps, lift the liver towards the animal head. By gently pulling the portion of the small intestine, fix the two lateral ends with a 6-0 polypropylene suture for a better view of the distal portion of the common bile duct tube.
Using a microvessel clip, occlude the proximal common bile duct temporarily. Expose the organ out of the abdominal cavity. Then, make a temporary occlusion of the distal common bile duct with an 8-0 suture. To access the common bile duct, puncture the periampullary region, which appears as a whitish part of the small intestine's wall, with a 0.4-millimeter needle connected to a 0.54-millimeter polyethylene tube. Start the infusion pump for 2.5% sodium taurocholate solution infusion at a constant speed of 10 microliters per 10 grams of body weight for 3 minutes.
After the infusion, remove the microvessel clip, the temporary 8-0 suture, and the injection needle from the pancreatic bile duct. Suture the abdomen with a 6-0 non-absorbent monofilament polypropylene suture. The time between the laparotomy and the end suture should not be more than 30 minutes. After the surgery, house the animals in polyethylene boxes lined with wood shavings and water and food ad libitum.
