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An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase

Article DOI: 10.3791/2094-v • 08:09 min • December 19th, 2010
December 19th, 2010 •
1, 1
1Department of Biochemistry, University of Toronto

Kapitel

Summary
Automatic Translation

The activity of the inducible lysine decarboxylase is monitored by reacting the substrate L-lysine and the product cadaverine with 2,4,6-trinitrobenzensulfonic acid to form adducts that have differential solubility in toluene.

Tags

Assay Measuring Activity Escherichia Coli Inducible Lysine Decarboxyase PH Acidic PH Survival Amino Acid Decarboxylases Glutamic Acid Decarboxylases Arginine Decarboxylase Lysine Decarboxylase Ornithine Decarboxylase Pyridoxal-5'-phosphate Co-factor Inner-membrane Substrate-product Antiporters Lysine-cadaverine Antiporter CadB X-ray Crystal Structure Stringent Response Regulator Guanosine 5'-diphosphate 3'-diphosphate PpGpp Stringent Response
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