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Journal / Immunology and Infection / Assessing Stomatal Response to Live Bacterial…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
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Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging

Article DOI: 10.3791/2185-v • 07:04 min • October 2nd, 2010
October 2nd, 2010 •
1, 1
1Biology, University of Texas at Arlington

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Summary
Automatic Translation

We have developed a simple and reproducible protocol to access stomatal response to live bacteria. This method minimizes wounding and manipulation of the leaf as compared to the use of epidermal peels reported previously.

Tags

Stomatal Response Live Bacterial Cells Whole Leaf Imaging Guard Cells Stomatal Pore Gas Exchange Light Intensity Carbon Dioxide Concentration Relative Humidity Pathogen Entry Disease Development Arabidopsis Plants Plant Innate Immunity Epidermal Peels Environmental Conditions MES Buffer Electrophysiological Experiments Bacterial Suspension
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