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Transformation of Plasmid DNA into E. coli Using the Heat Shock Method
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JoVE Journal Biologie
Transformation of Plasmid DNA into E. coli Using the Heat Shock Method

Transformation of Plasmid DNA into E. coli Using the Heat Shock Method

63,666 Views

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07:46 min

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August 01, 2007

DOI:

10.3791/253-v

DOI:
10.3791/253-v

•

07:46 min
August 01, 2007

•

216 Views
,
1Department of Physiology and Biophysics,University of California, Irvine (UCI)

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TransformationPlasmid DNAE. ColiHeat Shock MethodMolekularbiologieForeign PlasmidLigation ProductChemically Competent BacteriaGenlantisIncubationIceDNAHeat ShockSOC MediaTransformed CellsAgitationColoniesTransformation EfficiencyCompetent BacteriaTurbocells3-minute Transformation Protocol

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Froger, A., Hall, J. E. Transformation of Plasmid DNA into E. coli Using the Heat Shock Method. J. Vis. Exp. (6), e253, doi:10.3791/253 (2007).

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