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Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Article DOI: 10.3791/3322-v • 15:18 min • September 25th, 2011
September 25th, 2011 •
1, 1, 2, 3, 4, 1
1School of Molecular Bioscience, University of Sydney, 2Department of Surgery, Royal Prince Alfred Hospital, 3Department of Anatomical Pathology, Department of Anatomical Pathology, 4Department of Medicine, Concord Repatriation General Hospital

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Summary
Automatic Translation

We described a procedure for the disaggregation of colorectal cancer (CRC) to produce viable single cells, which are then captured on customized antibody microarrays recognizing surface antigens (DotScan CRC microarray). Sub-populations of cells bound to the microarray can be profiled by fluorescence multiplexing using monoclonal antibodies tagged with fluorescent dyes.

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Colorectal Cancer Cell Surface Protein Profiling Antibody Microarray Fluorescence Multiplexing CRC Prognosis Protein Expression Post-translational Modification Cluster Of Differentiation Antigens Biomarkers Immunohistochemistry (IHC) Cancer Sub-classification Prognostication Marker Clinico-pathological Staging Proteomic Techniques ITRAQ Diagnostic Laboratories
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