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Journal / Neuroscience / The Neuroblast Assay: An Assay for the Generation …
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JoVE Journal Neuroscience
The Neuroblast Assay
Examining Local Network Processing using Multi-contact Laminar Electrode Recording
Journal (Neuroscience)
Examining Local Network Processing using Multi-contact Laminar Electrode Recording
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The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry

Article DOI: 10.3791/3712-v • 15:41 min • April 22nd, 2012
April 22nd, 2012 •
1,2, 1, 1, 1
1Department of Neurosurgery, The University of Florida, 2Laboratory for Stem Cell Research, Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

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Summary
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This video protocol demonstrates a novel method for the generation and subsequent purification of neuronal progenitor cells from a renewable source of neural stem cells (NSCs) based on their physical (size and internal granularity) and fluorescent properties using flow cytometry technology.

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Neuroblast Assay Generation Of Neuronal Progenitor Cells Differentiating Neural Stem Cells Flow Cytometry Neurosphere Assay Central Nervous System Astrocytes Oligodendrocytes Neurons In Vitro Studies Drug Screening Neurotoxicology Electrophysiology Cell Replacement Therapy Heterogeneity Of NSC Progeny Low Production Of Neurons And Oligodendrocytes Predominance Of Astrocytes Clinical Applications Purification Of Immature Neurons Fluorescence Activated Cell Sorting (FACS) Murine NSC Progeny E14 Mouse Ganglionic Eminences
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