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Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
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Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

Article DOI: 10.3791/3747-v • 11:59 min • February 29th, 2012
February 29th, 2012 •
1, 1, 1, 1
1MRC Centre for Synaptic Plasticity, University of Bristol

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Summary
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This report describes the use of live cell imaging and photobleach techniques to determine the surface expression, transport pathways and trafficking kinetics of exogenously expressed, pH-sensitive GFP-tagged proteins at the plasma membrane of neurons.

Tags

Lateral Diffusion Exocytosis Membrane Proteins Cultured Neurons Fluorescence Recovery Fluorescence-loss Photobleaching Trafficking Receptors Ion Channels Synaptic Proteins Super-ecliptic PHluorin Live Cell Imaging Plasma Membrane Proteins Protonation Photon Absorption Fluorescence Emission Intracellular Trafficking Events Low PH Compartments Neutral PH Extracellular Environment Photodamage Photobleaching FRAP (fluorescence Recovery After Photobleaching) Protein Dynamics
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