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Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

Article DOI: 10.3791/3804-v • 08:25 min • April 3rd, 2012
April 3rd, 2012 •
1, 1, 1
1Yale Stem Cell Center, Department of Genetics, Yale School of Medicine

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Summary
Automatic Translation

A method to generate human induced pluripotent stem cells (iPSCs) via retrovirus-mediated ectopic expression of OCT4, SOX2, KLF4 and MYC is described. A practical way to identify human iPSC colonies based on GFP expression is also discussed.

Tags

Reprogramming Human Somatic Cells Induced Pluripotent Stem Cells Retroviral Vector GFP Human Embryonic Stem Cells Pluripotent In Vitro Disease Modeling Regenerative Medicine Transcription Factors Oct4 Sox2 Klf4 Myc Autologous Cells Fibroblast Cells GFP-containing Retroviral Backbone ESC Culture Condition Morphology Retroviral Transgene Silencing Fluorescence Microscope Pluripotency Genes Surface Markers
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