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JoVE Journal
Medicine
Cholesterin Efflux-Assay
Cholesterin Efflux-Assay
JoVE Journal
Medicine
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JoVE Journal Medicine
Cholesterol Efflux Assay

Cholesterin Efflux-Assay

Full Text
30,284 Views
07:54 min
March 6, 2012

DOI: 10.3791/3810-v

Hann Low1, Anh Hoang1, Dmitri Sviridov1

1Baker IDI Heart and Diabetes Institute

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Please note that some of the translations on this page are AI generated. Click here for the English version.

Summary

Die Cholesterin-Assay wurde entwickelt, um die Geschwindigkeit der Cholesterin-Efflux aus kultivierten Zellen und die Kapazität des Plasmas Akzeptoren, Cholesterin aus den Zellen freigesetzt akzeptieren zu quantifizieren. Der Test besteht aus Markieren von Zellen mit Cholesterin, Äquilibrierung von Cholesterin unter den intrazellulären Pools und Freisetzung von Cholesterol an einer extrazellulären Akzeptor.

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