Journal
/
Immunologie und Infektion
/
Real-time Live Imaging of T-cell Signaling Complex Formation
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Real-time Live Imaging of T-cell Signaling Complex Formation

Real-time Live Imaging of T-cell Signaling Complex Formation

DOI:
10.3791/50076-v

•

10:31 min

•

June 23, 2013

•

, ,
1The Mina and Everard Goodman Faculty of Life Sciences,Bar-Ilan University

Kapitel

  • 00:05Titel
  • 01:28T-cell Spreading Assay Preparation
  • 02:52T-cell Activation and Imaging
  • 07:21Imaging Data Analysis
  • 08:42Results: Live Imaging of SLP76 Deficient T-cells
  • 09:48Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We describe a live-cell imaging method that provides insight into protein dynamics during the T-cell activation process. We demonstrate the combined usage of the T-cell spreading assay, confocal microscopy and imaging analysis to yield quantitative results to follow signaling complex formation throughout T-cell activation.

Tags

T-cell SignalingImmune SystemT LymphocytesT-cell ActivationT-cell Antigen Receptor (TCR)Antigen Presenting Cells (APCs)Immune SynapseProtein-protein InteractionsLive Cell ImagingProtein Complex FormationReal-time ImagingJurkat CellsFluorescent Proteins

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System

Read Article