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JoVE Journal
Biology

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Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
 

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

Article DOI: 10.3791/50180-v 16:07 min March 30th, 2013
March 30th, 2013

Kapitel

Summary

We present robust biochemical and microscopic methods for studying Caenorhabditis elegans lipid stores. A rapid, simple, fixing-staining procedure for fluorescent lipid droplet imaging leverages the spectral properties of the lipophilic dye Nile red. We then present biochemical measurement of triglycerides and phospholipids using solid phase extraction and gas chromatography-mass spectrometry.

Tags

Keywords: Caenorhabditis Elegans Lipid Droplets Nile Red Staining High-throughput Microscopy Triglycerides Phospholipids Gas Chromatography-mass Spectrometry
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