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Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

Article DOI: 10.3791/50826-v • 09:20 min • December 21st, 2013
December 21st, 2013 •
1, 1, 1,2, 1,2
1Developmental Biology Institute of Marseille-Luminy (IBDML), Aix Marseille University, 2Campus de la Timone, European Research Center for Medical Imaging (CERIMED)

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Summary
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We describe a method for implanting and maintaining glass windows over the exposed spinal cords of adult mice for studying experimental autoimmune encephalomyelitis. These windows provide chronic optical access to the spinal cord for monitoring cell dynamics at the subcellular level in live animals using two-photon microscopy.

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Implanting Glass Spinal Cord Windows Adult Mice Experimental Autoimmune Encephalomyelitis Autonomic Demyelinating Diseases Multiple Sclerosis Intravital Microscopy Spinal Cord Cytoarchitecture Subcellular Resolution Low-cost Glass Window Implantation Protocol Surgical Exposure Of Vertebrae T12-L2 Chamber Construction With Cyanoacrylate And Dental Cement Laminectomy From T13 To L1 Transparent Silicone Elastomer Application Modified Glass Cover Slip Implantation Anti-inflammatory Treatment EAE Induction After Anti-inflammatory Treatment Cessation Thy1-CFP-23 Mice Axonal Loss Quantification
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