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Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair
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JoVE Journal Biologie
Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair

Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair

DOI:
10.3791/51106-v

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16:44 min

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March 24, 2014

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, ,
1Center for Genetic Medicine Research,Children’s National Medical Center, 2Department of Integrative Systems Biology,George Washington University

Kapitel

  • 00:05Titel
  • 01:53Imaging Cell Membrane Repair: Bulk Wounding
  • 04:59Live Imaging: Kinetics of Cell Membrane Repair
  • 06:23Imaging Bulk Injury Induced Lysosomal Exocytosis
  • 09:18Live Imaging of Cell Membrane Injury Triggered Subcellular Trafficking
  • 10:52Results: Single Cell Imaging of Injury and Repair
  • 15:58Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The process of healing injured cells involves trafficking of specific proteins and subcellular compartments to the site of cell membrane injury. This protocol describes assays to monitor these processes.

Tags

Cell Membrane InjuryCell Membrane RepairCell HealingSubcellular ProcessesLysosomal ExocytosisTIRF MicroscopyPulsed Laser InjuryCell CultureEnd-point Based AssayReal-time Imaging

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