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Journal / Bioengineering / Microfluidic plattform for måling Neutrofil…
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Bioengineering

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JoVE Journal Bioengineering
Microfluidic plattform for måling Neutrofil Chemotaxis fra Ubehandlet Whole Blood
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Microfluidic plattform for måling Neutrofil Chemotaxis fra Ubehandlet Whole Blood

Article DOI: 10.3791/51215-v • 10:13 min • June 3rd, 2014
June 3rd, 2014 •
*1,2,3, *1,2,3, 1, 1, 1,4, 1,2,3
1The BioMEMS Resource Center, Department of Surgery, Massachusetts General Hospital, 2Harvard Medical School, 3Shriners Burns Hospital, 4Harvard University School of Engineering and Applied Sciences
* These authors contributed equally

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Denne protokollen detaljer en assay utviklet for å måle human neutrofil kjemotakse av en dråpe av helblod med robust reproduserbarhet. Denne tilnærmingen omgår behovet for nøytrofile separasjon og krever bare noen få minutter av analysen forberedelsestid. Den microfluidic brikken gjør det mulig for den gjentatte mål på nøytrofil-kjemotakse over tid hos spedbarn og små pattedyr, hvor prøvevolum er begrenset.

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Bioteknologi kjemotaksis nøytrofile fullblod analysen microfluidic enhet kjemoattraktant migrasjon betennelse
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