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Brain Slice Biotinylation
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Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

Article DOI: 10.3791/51240-v • 06:18 min • April 3rd, 2014
April 3rd, 2014 •
1, 1, 2
1Program in Neuroscience, Graduate School of Biomedical Sciences, University of Massachusetts Medical School, 2Brudnick Neuropsychiatric Research Institute, Department of Psychiatry, University of Massachusetts Medical School

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Summary
Automatic Translation

Neuronal membrane trafficking dynamically controls plasma membrane protein availability and significantly impacts neurotransmission. To date, it has been challenging to measure neuronal endocytic trafficking in adult neurons. Here, we describe a highly effective, quantitative method to measure rapid changes in surface protein expression ex vivo in acute brain slices.

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Brain Slice Biotinylation Ex Vivo Approach Plasma Membrane Protein Trafficking Adult Neurons Endocytic Trafficking Surface Biotinylation Membrane Protein Surface Expression Exogenous Stimuli Gene Manipulation Cultured Cells Region-specific Differences Acute Brain Slice Preparation Rapid Changes High-fidelity Approach Neuronal Endocytic Trafficking
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