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Hot Biological Catalysis

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Article DOI: 10.3791/51487-v 13:00 min April 4th, 2014
April 4th, 2014



Isothermal titration calorimetry measures heat flow released or absorbed in chemical reactions. This method can be used to quantify enzyme-catalysis. In this paper, the protocol for instrumental setup, experiment running, and data analysis is generally described, and applied to the characterization of enzymatic urea hydrolysis by jack bean urease.


Keywords: Isothermal Titration Calorimetry ITC Enzymatic Reactions Enzyme Kinetics Thermodynamics Kcat KM Enthalpy Urease Jack Bean
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