Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Rapid Isolation And Purification Of Mitochondria…
JoVE Journal
Biology

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können.

JoVE Journal Biology
Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration
Episomal Reprogramming of Dermal Fibroblasts to Generate Human-Induced Pluripotent Stem Cells (HiPSCs) (Video) | JoVE
Journal (Biology)
Episomal Reprogramming of Dermal Fibroblasts to Generate Human-Induced Pluripotent Stem Cells (HiPSCs) (Video) | JoVE
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
Journal (Biology)
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
 

Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration

Article DOI: 10.3791/51682-v • 10:35 min • September 6th, 2014
September 6th, 2014 •
1, 2, 1, 2, 2, 1
1Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, 2Department of Anesthesiology, Perioperative and Pain Medicine, Boston Children's Hospital and Department of Anesthesia, Harvard Medical School

Kapitel

Summary
Automatic Translation

A method for rapid isolation of mitochondria from mammalian tissue biopsies is described. Rat liver or skeletal muscle preparations were homogenized with a commercial tissue dissociator and mitochondria were isolated by differential filtration through nylon mesh filters. Mitochondrial isolation time is <30 min compared to 60 - 100 min using alternative methods.

Tags

Rapid Isolation Purification Mitochondria Transplantation Tissue Dissociation Differential Filtration Centrifugation Ficoll Gradient Centrifugation Commercial Tissue Dissociator Homogenization Nylon Mesh Filters Viable Mitochondria Respiration Competent Mitochondria
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter