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Chemistry

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Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
 

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Article DOI: 10.3791/51840-v 13:06 min November 20th, 2014
November 20th, 2014

Kapitel

Summary

In order to comprehensively explore the diversity of O-linked glycans, a new procedure for in-gel reductive β-elimination, combined with permethylation and a rapid phase-partition method, is applied to the analysis of O-linked glycans directly released from glycoproteins resolved by SDS-PAGE and amenable to subsequent glycomic analysis by mass spectrometry.

Tags

Keywords: In-gel Reductive β-elimination O-linked Glycans Sulfo-glycans SDS-PAGE Mass Spectrometry Glycomic Analysis Sulfoglycomic Analysis Glycoproteins Permethylation Phase-partition
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