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RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
DOI:

11:13 min

November 01, 2014

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Kapitel

  • 00:05Titel
  • 01:22Harvesting Neural Tubes for RNA Collection
  • 04:32Pooling Tissue to Purify RNA for Illumina Sequencing
  • 05:34Comparing RNA-Seq Datasets on the Galaxy Public Server
  • 09:52Results: Transcriptome Analyis Quality
  • 09:52Transcriptome Analysis Quality
  • 10:42Conclusion

Summary

Automatische Übersetzung

This video shows the basic steps for performing whole transcriptome analysis on dissected chick embryonic spinal cord samples after transfection with in ovo electroporation.

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