Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Amanda M. Larracuente; Patrick M. Ferree

doi:10.3791/52288

Journal

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Biologie

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Einfache Methode zur Fluoreszenz DNA In-situ- Die Hybridisierung an Squashed Chromosomen

Simple Method for Fluorescence DNA <em>In Situ</em> Hybridization to Squashed Chromosomes

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Biologie

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JoVE Journal Biologie

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Einfache Methode zur Fluoreszenz DNA In-situ- Die Hybridisierung an Squashed Chromosomen

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18,022 Views

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11:36 min

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January 06, 2015

DOI:

10.3791/52288-v

DOI:

10.3791/52288-v

•

11:36 min

January 06, 2015

•

7 Views

Amanda M. Larracuente¹, Patrick M. Ferree²

¹Department of Biology,University of Rochester, ²W. M. Keck Science Department,Claremont McKenna, Pitzer, and Scripps Colleges

Summary

Here, we present a simple method for performing fluorescence DNA in situ hybridization (DNA ISH) to visualize repetitive heterochromatic sequences on slide-mounted chromosomes. The method requires minimal reagents and it is versatile for use with short or long probes, different tissues, and detection with fluorescence or non-fluorescence-based signals.