Effiziente Erzeugung von hiPSC Neural Lineage Spezifische Knockin Reporter Mit den CRISPR / Cas9 und Cas9 Doppel Nickase-System

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Zitiert von 5

14:46 Min.

28. Mai 2015

10.3791/52539-v

28. Mai 2015

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Shenglan Li^*¹^,² , Haipeng Xue^*¹^,² , Bo Long¹^,²^,⁵ , Li Sun¹^,²^,⁶ , Tai Truong¹^,²^,⁴^,⁷ , Ying Liu¹^,²^,³

¹Department of Neurosurgery, The University of Texas Health Science Center at Houston, ²Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, ³The Senator Lloyd & B. A. Bentsen Center for Stroke Research, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, ⁴Summer Research Program, Office of Educational Programs, The University of Texas Health Science Center at Houston, ⁵Department of Anesthesiology, Shengjing Hospital, China Medical University, ⁶Department of Oncology, Renji Hospital, Shanghai Jiaotong University School of Medicine, ⁷Biology Department, University of West Georgia

^* These authors contributed equally

Genom-Editing-Tools wie das CRISPR-System (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) haben die Effizienz des Gen-Targetings in humanen induzierten pluripotenten Stammzellen (hiPSCs) erheblich verbessert. Dieses Manuskript beschreibt ein Protokoll zur Generierung eines linienspezifischen hiPSC-Reporters unter Verwendung der CRISPR/Cas-System-unterstützten homologen Rekombination.