Isolation von Leukozyten aus dem menschlichen Mutter und Fötus-Schnittstelle
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May 21, 2015
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Described herein is a protocol to isolate and further study the infiltrating leukocytes of the decidua basalis and decidua parietalis - the human maternal-fetal interface. This protocol maintains the integrity of cell surface markers and yields enough viable cells for downstream applications as proven by flow cytometry analysis.
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Cite this Article
Xu, Y., Plazyo, O., Romero, R., Hassan, S. S., Gomez-Lopez, N. Isolation of Leukocytes from the Human Maternal-fetal Interface. J. Vis. Exp. (99), e52863, doi:10.3791/52863 (2015).
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