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Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
JoVE Journal
Biologie
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JoVE Journal Biologie
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

16,846 Views

09:04 min

August 17, 2015

DOI:

09:04 min
August 17, 2015

6 Views
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Summary

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This protocol describes how to perform absolute quantification assays of target proteins within complex biological samples using selected reaction monitoring. It was used to accurately quantify proteins of the mouse macrophage chemotaxis signaling pathway. Target peptide selection, assay development, and qualitative and quantitative assays are described in detail.

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