Überwachung Endoplasmatischen Reticulum Calciumhomöostase Mit ein<em> Gaussia</em> Luciferase SERCaMP
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September 06, 2015
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Summary
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Endoplasmic reticulum calcium homeostasis is disrupted in diverse pathologies. A secreted ER calcium monitoring protein (SERCaMP) reporter can be used to detect disruptions in the ER calcium store. This protocol describes the use of a Gaussia luciferase SERCaMP to examine ER calcium homeostasis in vitro and in vivo.
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Henderson, M. J., Wires, E. S., Trychta, K. A., Yan, X., Harvey, B. K. Monitoring Endoplasmic Reticulum Calcium Homeostasis Using a Gaussia Luciferase SERCaMP. J. Vis. Exp. (103), e53199, doi:10.3791/53199 (2015).
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