Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

Sarah J. Potter; Tony DeFalco

doi:10.3791/53262

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Verwendung Ex Vivo Upright Droplet Kulturen von Whole Fetal Orgeln zu Entwicklungsprozessen während der Maus Organogenese Studieren

Using <em>Ex Vivo</em> Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

JoVE Journal
Entwicklungsbiologie

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JoVE Journal Entwicklungsbiologie

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

Verwendung Ex Vivo Upright Droplet Kulturen von Whole Fetal Orgeln zu Entwicklungsprozessen während der Maus Organogenese Studieren

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12,421 Views

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09:47 min

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October 21, 2015

DOI:

10.3791/53262-v

DOI:

10.3791/53262-v

•

09:47 min

October 21, 2015

•

14 Views

Sarah J. Potter¹, Tony DeFalco¹

¹Division of Reproductive Sciences,Cincinnati Children’s Hospital Medical Center

Summary

The ex vivo upright droplet culture is an alternative to current in vitro and in vivo experimental techniques. This protocol is easy to perform and requires smaller amounts of reagent, while permitting the ability to manipulate and study fetal vascularization, morphogenesis, and organogenesis.