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A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting

Article DOI: 10.3791/53409-v • 05:34 min • November 20th, 2015
November 20th, 2015 •
1, 2, 1, 1
1Lombardi Comprehensive Cancer Center, Department of Oncology, Georgetown University, 2Department of Biomedical Sciences, University of Minnesota

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Summary
Automatic Translation

This method evaluates cancer cell invasion from spheroids into a surrounding 3D matrix. Spheroids are generated via the hanging drop culture method and then embedded in a matrix comprised of basement membrane materials and type I collagen. Invasion out of the spheroids is subsequently monitored.

Tags

Cancer Cell Spheroid Assay Invasion 3D Setting Metastatic Potential Traditional Assays Invasive Features Three-dimensional Environment Reduced Biological Applicability Physiologically Relevant Setting Gravity Spheroids Drops Of Media Cell Culture Dish 3D Matrix Basement Membrane Materials Type I Collagen Cancer Cell Egression Surrounding Matrix Coculture Of Different Cell Types Drugs/inhibitors Extracellular Matrix (ECM) Constituents
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