Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Alison Gaylo; Michael G. Overstreet; Deborah J. Fowell

doi:10.3791/53585

Imaging CD4 T-Zell-Interstitial Migration in den entzündeten Dermis

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Imaging CD4 T-Zell-Interstitial Migration in den entzündeten Dermis

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11:28 min

March 25, 2016

DOI: 10.3791/53585-v

Alison Gaylo¹, Michael G. Overstreet¹, Deborah J. Fowell¹

¹David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology,University of Rochester School of Medicine and Dentistry, Rochester, NY

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Overview

This article presents a non-invasive imaging protocol to visualize CD4 positive T cell dynamics in inflamed skin. The method allows researchers to study the mechanisms underlying immune cell motility and function in situ.

Key Study Components

Area of Science

Immunology
Cell Biology
Inflammation Research

Background

Understanding CD4 effector T cell behavior is crucial in immunology.
Current knowledge of T cell motility in inflamed tissues is limited.
Non-invasive techniques are needed for better visualization.
Proper positioning of the ear is essential for high-quality imaging.

Purpose of Study

To visualize CD4 T cell dynamics in the inflamed ear dermis.
To explore the mechanisms of immune cell motility.
To provide a minimally invasive method for studying T cell behavior.

Methods Used

Aspirating fluorescently labeled effector T cell suspension.
Using a syringe equipped with a fine gauge needle for injection.
Preparing the ear for imaging to ensure accurate results.
Visual demonstration of the protocol for clarity.

Main Results

The imaging protocol successfully visualizes T cell dynamics.
Demonstrated the importance of ear positioning for imaging quality.
Provided insights into T cell behavior in inflamed tissues.
Highlighted the advantages of a non-invasive approach.

Conclusions

The method offers a new way to study immune cell dynamics.
It can help answer critical questions in immunology.
Future research can build on this technique to explore T cell functions.

Frequently Asked Questions

What is the main goal of this imaging protocol?

The main goal is to visualize CD4 positive T cell dynamics in inflamed skin.

Why is this method considered minimally invasive?

It allows for the study of T cell behavior without extensive surgical procedures.

What are the key challenges in preparing the ear for imaging?

Proper positioning of the ear is critical for capturing high-quality images.

Who is demonstrating the protocol?

Alison Gaylo, a graduate student, is conducting the demonstration.

How does this research contribute to immunology?

It provides insights into the mechanisms of immune cell motility in inflamed tissues.

What are the advantages of this imaging technique?

It offers a non-invasive way to study dynamic behavior of T cells in situ.

Die Mechanismen, die die interstitielle Motilität von CD4 Effektor T-Zellen an Entzündungsstellen bestimmen, sind relativ unbekannt. Wir stellen eine nicht-invasive Ansatz zu visualisieren und in vitro -geprimte CD4 - T - Zellen in den entzündeten Ohr Dermis, so dass für das Studium des dynamischen Verhaltens dieser Zellen in situ zu manipulieren.

Das übergeordnete Ziel dieses Bildgebungsprotokolls ist es, die CD4-positive T-Zell-Dynamik in der entzündeten Haut sichtbar zu machen. Diese Methode kann dazu beitragen, Schlüsselfragen auf dem Gebiet der Immunologie zu beantworten, z. B. welche Mechanismen der Beweglichkeit und Funktion von Immunzellen in entzündeten Geweben zugrunde liegen. Der Hauptvorteil dieser Technik besteht darin, dass sie eine minimal-invasive Möglichkeit bietet, das dynamische Verhalten von CD4-T-Zellen in Situ zu untersuchen.

visuelle Demonstration dieser Methode ist von entscheidender Bedeutung, da die Schritte zur Vorbereitung des Ohrs für die Bildgebung schwer zu erlernen sind und die richtige Positionierung des Ohrs erforderlich ist, um qualitativ hochwertige Bilder aufzunehmen. Ich bin Alison Gaylo, eine Doktorandin, und ich werde die Demonstration dieses Protokolls durchführen. Beginnen Sie mit der Aspiration von 300 Mikrolitern fluoreszenzmarkierter Effektor-T-Zell-Suspension pro Maus in eine Spritze, die mit einer 27,5-Gauge-Nadel ausgestattet ist.

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